Combination of Bovine Carbonic Anhydrase with a Fluorescent Sulfonamide
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چکیده
منابع مشابه
Combination of bovine carbonic anhydrase with a fluorescent sulfonamide.
Bovine erythrocyte carbonic anhydrase forms a highly fluorescent complex with 5-dimethylaminonapbthalene-lsulfonamide (DNSA). The binding, studied either by enhancement of ligand fluorescence or by the quenching of protein ultraviolet fluorescence, shows that only 1 mole of DNSA is bound per mole of protein; the dissociation constant at pH 7.4 is 2.5 X lo-’ M. The fluorescence of free DNSA in w...
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Microcalorimetry is an excellent method of determining the thermodynamics of ligand–protein binding in solution, because the signal being measured is the observed binding enthalpy (Db-obsH [Editors’ note: this term can also be written DHobs or DbHobs as seen elsewhere in this book]), which is free of any model assumptions. The affinity (DbG) can be computed from the shape of a binding curve, an...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99374-9